Staphylococcus one-minute and after five minutes contact time was

Staphylococcus aureus and coagulase-negative staphylococci (CNS) are common causes of bovine and caprine intermammary infections. S. aureus infections, which can be clinical or subclinical, frequently persist for a long time, and infected mammary glands thus serve as reservoirs from which the organism may spread to other cows within a herd and occasionally to other herds 21. A main challenge in food industry is to avoid contamination of raw materials and products by pathogens and spoilage organisms by controlling of microorganisms on food contact surfaces such as milking machine, milking utensils and dairy equipment 22.The results in tables (5&6) fig. (1&2) showed that the logarithmic reduction of average bacterial count of the four species of antibiotic resistance coagulase negative staphylococci tested up on addition of Dyne-O-Might 1% after contact time one-minute and after five minutes contact time was not achieved and not considered bactericidal according to the standard used except for Staphylococcus hyicus after five minutes contact time as the log reduction was 6.5. while other strains fail to response to this disinfectants this may be due to antibiotic resistance gene. Our results were coinciding to certain limit to those obtained by Boddie et al 23 and disagreed with McLure &Gordon 24 who found that, iodine exhibited a superior killing effect on 33 clinical isolates of methicillin-resistant Staphylococcus aureus when measured by rate of kill or final logarithmic reduction factors (LRFs ) achieved while Elisabeth et al. 25 mentioned that , iodine led to a log reduction of the viable cells of Staphylococcus epidermidis of >5 after incubation for 5 min . 0.2% povidone solution reduced methicillin-resistant Staphylococcus aureus under detection limits at 37, 24 hrs incubation and 7.5 % povidone-iodine, were most effective reducing MRSA under detection limits. (less than 10 CFU/ml) 26.Concerning non chlorine releasing agent Zixvirox 0.5%, The required 5 log reduction in the suspension test was achieved even after short contact time one minute. These results agreed with Elisabeth et al. 25 who found that, Hydrogen peroxide, at a concentration of 3% and 5%, rapidly eradicate Staphylococcus epidermidis biofilms, whereas povidone-iodine is less effective, H2O2 demonstrates broad-spectrum efficacy against viruses, bacteria, yeasts, and bacterial spores. Leslie et al. 27 mentioned that, hydrogen peroxide-based teat disinfectant provided significant improvement in teat skin condition and no adverse effects on teat end condition. Peracetic acid is considered a more potent biocide than hydrogen peroxide, being sporicidal, bactericidal, virucidal, and fungicidal at low concentrations (<0.3%) 28. These results may be due to the combination of peracetic acid and hydrogen peroxide was found to be synergistic. And that synergy was maintained with increasing contact time 29. On the other hand, detergent agent like Polycare 3%was not considered bactericidal agent as the log reduction was lower than 5 even after five minutes contact time, Polycare is a surfactant used for cleaning purpose has low to moderate foaming ability and not considered bactericidal agent. Salah et al., 22 found that quaternary ammonium compound was not achieved the required log reduction even after 30 minutes' contact time when tested against staphylococcus aureus. So Polycare could be used as primary cleaning agent not effective disinfectantRegarding addition of virkon S 1%, table (5) showed that, the log. reduction was greater than 5 after 5 minutes contact time for all tested strains except Staphylococcus intermedius the log reduction was 7.1 after one minute and 8.1 after 5 minutes.It could be referred to the resistant Antibiogramme they possess . Patterson et al. 30 concluded that, 4% peroxymonosulfate solution was successful in reducing counts of bacterial CFUs of S. aureus by > 99.9999%. Meanwhile Dunowska et al. 31 evaluate the efficacy of aerial disinfection using 1% virkon S and found that, The reduction of S. aureus counts ranged from 4.92 to 0.02 log (10).The01234567Dyne-O-Might 1%Zixvirox 0.5%Polycare 3%Virkon 1%Log reductioFig 3 staphylococcus Log reduction of surface test after five minutes contactS. intermediusS. xylosusS. hyicusS. sciuriEffect of Some Disinfectants on Antibiotic Resistance Staphylococcus Isolated from Dairy Farms…DOI: 10.9790/2380-081210107 www.iosrjournals.org 6 | Pagebactericidal activity of virkon may be due to the other ingredients which present with virkon like mallic acid ,these acid increase the acidity of virkon making it work better.The results of surface test were cleared in table 7, fig. 3; the logarithmic reduction was somewhat lower than that obtained by suspension test. This may be due to the surface nature play the most important role and the destruction rate when the cells are in suspension is higher in this condition than when the microorganisms are settled on a surface 32, The variation of results between strain on applying surface and suspension test and within each test may have attributed to resistance antibiogram